Evidence for Acquisition in Nature of a Chromosomal 2,4-Dichlorophenoxyacetic Acid/a-Ketoglutarate Dioxygenase Gene by Different Burkholderia spp

Evidence for Acquisition in Nature of a Chromosomal 2,4-Dichlorophenoxyacetic Acid/(alpha)-Ketoglutarate Dioxygenase Gene by Different Burkholderia spp.

We characterized the gene required to initiate the degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) by the soil bacterium Burkholderia sp. strain TFD6, which hybridized to the tfdA gene of the canonical 2,4-D catabolic plasmid pJP4 under low-stringency conditions. Cleavage of the ether bond of 2,4-D by cell extracts of TFD6 proceeded by an (alpha)-ketoglutarate-dependent reaction, characte...

Characterization of a chromosomally encoded 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase from Burkholderia sp. strain RASC.

The findings of previous studies indicate that the genes required for metabolism of the pesticide 2,4-dichlorophenoxyacetic acid (2,4-D) are typically encoded on broad-host-range plasmids. However, characterization of plasmid-cured strains of Burkholderia sp. strain RASC, as well as mutants obtained by transposon mutagenesis, suggested that the 2,4-D catabolic genes were located on the chromoso...

Characterization of a Chromosomally Encoded 2,4-Dichlorophenoxyacetic Acid/a-Ketoglutarate Dioxygenase from Burkholderia sp. Strain RASC

Partial Purification and Characterization of 2,4- Dichlorophenoxyacetic Acid Degrading Bacteria Harboring Alpha Ketoglutarate Dioxgenase Enzyme

The catabolic adaptability demonstrated by microorganisms is important in the bioremediation of polluted environments. 2,4-dichlorophenoxyacetic acid/α-ketoglutarate dioxygenase is the first enzyme in the catabolic pathway for the 2,4dichlorophenoxyacetic acid (2,4-D). In the present study, dioxygenase enzymes were inducible and the serial adaptation of four studied bacterial isolates steadily ...

Alcaligenes eutrophus JMP134 "2,4-dichlorophenoxyacetate monooxygenase" is an alpha-ketoglutarate-dependent dioxygenase.

The Alcaligenes eutrophus JMP134 tfdA gene, encoding the enzyme responsible for the first step in 2,4-dichlorophenoxyacetic acid (2,4-D) biodegradation, was overexpressed in Escherichia coli, and several enzymatic properties of the partially purified gene product were examined. Although the tfdA-encoded enzyme is typically referred to as 2,4-D monooxygenase, we were unable to observe any reduct...